培養基和添加劑 | RPMI-1640(GIBCO,貨號31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L)，90%；優質胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。 |
REFERENCE | Williams RD , et al. In vitro c*tion of human renal cell cancer. I. Establishment of cells in culture. In Vitro 12: 623-627, 1976. PubMed: 1010528 Williams RD , et al. In vitro c*tion of human renal cell cancer. II. Characterization of cell lines. In Vitro 14: 779-786, 1978. PubMed: 721102 Thiede MA , et al. Human renal carcinoma expresses two messages encoding a parathyroid hormone-like peptide: evidence for the alternative splicing of a single- copy gene. Proc. Natl. Acad. Sci. USA 85: 4605-4609, 1988. PubMed: 3290897 Shanghai Immunnol 22(3):189-190,2002 Chin J Immunol 18(11):766-768,2002 Chin J Urol 24(8):514-516,2003 |
