培養基和添加劑 | 訂購此細胞需提供100ml培養基。LHC-9培養液添加0.5鈉克/毫升重組上皮細胞生長因子(EGF),500鈉克/毫升氫*可的松，0.035鈉克/毫升牛垂體提取物，500mM乙醇胺，500nM 磷酸乙醇胺，0.01毫克/毫升腎上腺素，0.1鈉克/毫升retinoic酸及微量元素。注意：不要過濾EGF。LHC-9基礎培養液可以從Biofluids, Inc., Rockville, MD(目錄號118)或Clonetics Corporation, Walkersville, MD 21793(BEBM目錄 |
REFERENCE | Reddel RR , et al. Immortalized human bronchial epitherial mesothelial cell lines. US Patent 4,885,238 dated Dec 5 1989 Lechner JF , LaVeck MA . A serum-free method for culturing normal human bronchial epithelial cells at clonal density. J. Tissue Culture Methods 9: 43-48, 1985. Sakamoto O , et al. Role of macrophage-stimulating protein and its receptor, RON tyrosine kinase, in ciliary motility. J. Clin. Invest. 99: 701-709, 1997. PubMed: 9045873 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C. |
