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Rabbit Anti- IGFBP1 antibody
Catalog Number : BYK- 1822R
Quantity size : 0.1ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background: Insulin like growth factor binding protein 1 （IGFBP1） is a member of the superfamily of insulin like growth factor （IGF） binding proteins which include six high affinity IGF binding proteins （IGFBP） and at least four low affinity binding proteins referred to as IGFBP related proteins （IGFBPrP）. The IGFBP members are cysteine rich proteins with conserved cysteine residues clustered in the amino terminal and the carboxy terminal regions of the molecule. The N terminal and C terminal regions are highly homologous among rat, human and bovine sequences. Contained within IGFBP1 and 2 is an integrin receptor recognition sequence （RGD） that is responsible for promoting cell migration by an IGF independent action. IGFBPs hold a central position in IGF ligand receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment. IGFBPs will either inhibit or enhance the biological activities of IGF or act in an IGF independent manner. Post translational modification of IGFBPs, including phosphorylation and proteolysis, will modify the affinities of the binding proteins for IGF and may indirectly regulate IGF actions. IGFBP1 is expressed in liver, decidua, and kidneys and is the major IGF binding protein in human amnionic fluid. In hepatocytes, IGFBP1 production is regulated at the transcriptional level due to the effects of insulin and corticosteriods. IGFBP1 is the major determinant of the level of free IGF in serum. The expression of IGFBP1 is inhibited by insulin, IGFI, and IGFII and is stimulated by glucocorticoids, thyroid hormone, and epidermal growth factor （EGF）, indicating an endocrine function. IGFBP1 shows inhibitory actions on cell proliferation and differentiation, presumably by interfering with the interactions between IGF and the IGF receptor （IGFR）.
Anti-IGFBP1胰島素樣生長因子結(jié)合蛋白-1抗體說明書Specificity :
–Anti- IGFBP1 is a rabbit polyclonal antibody unconjugated
–specific for IGFBP1 of rat，hu，mo，rb
–use for western blotting,elisa,immunoprecipitation and immunohistochemistry
–Protein G affinity chromatography purification, purity :>95%
–Isotype: IgG
–mol wt: 30kDa
Application :
–Western blotting 1:100-500
–Immunohistochemistry 1:100-500
–ELISA 1:500-1000
–IP=1:20-100 IF=1:100-500
–Optimal working dilutions must be determined by the end user.
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Anti-IGFBP1胰島素樣生長因子結(jié)合蛋白-1抗體說明書Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
